Bruker Autoflex maX TOF/TOF MALDI Mass Spectrometer
Mass Range : up to 500,000
Resolution :
Linear Mode
Positive Ion Mode ≥ 1,100 @ m/z 12,361 (Cytochrome C)
Negative Ion Mode ≥ 2,500 @ m/z 1,569 (Glu-Fib)
Reflector Mode
Positive Ion Mode > 26,000 @ m/z 3,147 (Somatostatin)
Negative Ion Mode > 13,000 @ m/z 1568.66 Glu-Fib)
Mass accuracy: <2 (int. calib.) [ppm] Reflector mode
Laser
- BRUKER smartbeam™-II laser, 355 nm wavelength
- Minimum number of 3.5*109 laser shots
- Repetition rate up to 2 kHz
Laser frequency [Hz]: 2000 (MS) / 200 (TOF/TOF)
Ion Optics Effective Flight Length
Linear Mode 120 cm
Reflector Mode 215 cm
TOF/TOF-Acquisition:
Max. usable Laser speed 200 Hz
MS/MS Sensitivity [Glu1]-Fib fragment (m/z 1,056) @ S/N ≥ 10:1 for 250 amol shown on Bruker AnchorChip™ target with 2,000 laser shots
MS/MS Mass Accuracy Average Mass Error ≤ 0.05 Da for relevant fragments of [Glu1]-Fibrinopeptide B (m/z 1,570)
MS/MS fragment resolution ≥ 800 for [Glu1]-Fib fragment m/z 175
≥ 2,000 for [Glu1]-Fib fragment m/z 684
≥ 2,500 for [Glu1]-Fib fragment m/z 1,056
≥ 3,500 for [Glu1]-Fib fragment m/z 1,441
Pre Cursor Ion Selector Resolution Precursor ion selection has a resolution of ≥450.
MALDI autoFlex
Location: Hunter 241A
Contact: Dr. Rakesh Sachdeva