{"id":19,"date":"2026-03-09T14:59:49","date_gmt":"2026-03-09T14:59:49","guid":{"rendered":"https:\/\/scienceweb.clemson.edu\/sparc\/?page_id=19"},"modified":"2026-03-13T18:24:53","modified_gmt":"2026-03-13T18:24:53","slug":"sparc-overview","status":"publish","type":"page","link":"https:\/\/scienceweb.clemson.edu\/sparc\/","title":{"rendered":"SPARC | Spatial Profiling and Analyses Resources at Clemson"},"content":{"rendered":"

SPARC, led by<\/span> Dr. Shahid Mukhtar<\/a><\/strong> at Clemson University, serves as a Certified Service Provider (CSP) for<\/span> Complete Genomics<\/strong><\/a>.<\/span> Through this partnership, SPARC provides end-to-end spatial transcriptomics services using the Stereo-seq (SpaTial Enhanced<\/span> REsolution Omics sequencing) platform developed by<\/span> STOmics<\/a><\/strong>, a spatial biology division of Complete Genomics.\u00a0<\/span><\/h4>\n

Why spatial, why now<\/strong><\/h3>\n

Spatial omics captures gene expression in tissue context, enabling questions about cellular neighborhoods, microenvironments, and tissue architecture that cannot be answered with dissociation-only methods. Spatial technologies\u00a0 bridge morphology with molecular readouts, supported by dedicated workflows and analysis support.<\/span><\/h4>\n\n\n
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\"Perma\"<\/figure>\n<\/figure>\n<\/div>\n\n\n\n
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\"spatial<\/figure>\n<\/figure>\n<\/div>\n<\/div>\n\n\n\n
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\"Embryos<\/figure>\n<\/figure>\n<\/div>\n<\/div><\/div>\n\n\n\n
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Why Stereo-seq<\/strong><\/h3>\n

Stereo-seq\u00a0is a next-generation spatial transcriptomics technology that enables genome-wide mapping of gene expression directly within intact tissue sections. By capturing mRNA on densely patterned arrays of spatially barcoded DNA nanoballs, Stereo-seq preserves the physical context of gene activity while achieving extremely high spatial resolution across large tissue areas.\u00a0<\/span><\/h4>\n<\/div>\n\n\n\n
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\u00a0<\/h4>\n
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    Ultra-high spatial resolution<\/strong><\/span><\/h4>\n<\/li>\n
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    Whole-transcriptome coverage<\/strong><\/span><\/h4>\n<\/li>\n
  • \n

    Large tissue field of view<\/strong><\/span><\/h4>\n<\/li>\n
  • \n

    Powerful biological insight<\/strong><\/span>

    <\/h4>\n<\/li>\n<\/ul>\n<\/div>\n<\/div><\/div>\n\n\n

    \u00a0<\/h4>\n

    What SPARC offers<\/strong><\/h3>\n
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      Experimental design consultation\u00a0<\/strong><\/span><\/h4>\n
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        Guidance on experimental design and project planning<\/span><\/h4>\n<\/li>\n
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        Recommendations for tissue handling and sample preparation<\/span><\/h4>\n<\/li>\n
      • \n

        Feasibility assessment for proposed studies<\/span><\/h4>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n
          \n
        • \n

          \u00a0Spatial transcriptomics at high spatial resolution<\/strong><\/span><\/h4>\n
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            Tissue intake, quality assessment, and sample handling support<\/span><\/h4>\n<\/li>\n
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            Cryo-embedding, cryosectioning, and slide preparation<\/span><\/h4>\n<\/li>\n
          • \n

            Staining, histology support, and imaging<\/span><\/h4>\n<\/li>\n
          • \n

            Spatial transcriptomics workflow execution, including permeabilization and Stereo-seq<\/span><\/h4>\n<\/li>\n
          • \n

            Spatial library preparation and sequencing coordination<\/span><\/h4>\n<\/li>\n<\/ul>\n<\/li>\n
          • \n

            \u00a0Single-cell omics analysis<\/strong><\/span><\/h4>\n
              \n
            • \n

              Processing and analysis of single-cell RNA-seq and related omics datasets<\/span><\/h4>\n<\/li>\n
            • \n

              Cell clustering, cell-type identification, and annotation<\/span><\/h4>\n<\/li>\n
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              Integration of single-cell datasets with spatial transcriptomics data<\/span><\/h4>\n<\/li>\n
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              Generation of interpretable visualizations and reports for biological insight<\/span><\/h4>\n<\/li>\n<\/ul>\n<\/li>\n
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              Computational analysis<\/strong><\/span><\/h4>\n
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              • \n

                Generation of expression matrices and spatial gene expression maps<\/span><\/h4>\n<\/li>\n
              • \n

                Clustering and spatial domain identification<\/span><\/h4>\n<\/li>\n
              • \n

                Cell-type annotation and spatial mapping<\/span><\/h4>\n<\/li>\n
              • \n

                Standard visualizations and summary reports using established analysis workflows <\/span><\/h4>\n<\/li>\n
              • \n

                Advanced gene regulatory network (GRN analysis<\/span><\/h4>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n

                <\/p>\n\n\n\n

                \n
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                \"Bioinformatics<\/figure>\n<\/div>\n\n\n\n
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                \"Bioinformatics<\/figure>\n<\/div>\n<\/div><\/div>\n\n\n\n

                <\/p>\n","protected":false},"excerpt":{"rendered":"

                SPARC, led by Dr. Shahid Mukhtar at Clemson University, serves as a Certified Service Provider (CSP) for Complete Genomics. Through […]<\/p>\n","protected":false},"author":64,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-19","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"mshahid","author_link":"https:\/\/scienceweb.clemson.edu\/sparc\/author\/mshahid\/"},"uagb_comment_info":0,"uagb_excerpt":"SPARC, led by Dr. Shahid Mukhtar at Clemson University, serves as a Certified Service Provider (CSP) for Complete Genomics. Through […]","_links":{"self":[{"href":"https:\/\/scienceweb.clemson.edu\/sparc\/wp-json\/wp\/v2\/pages\/19","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scienceweb.clemson.edu\/sparc\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/scienceweb.clemson.edu\/sparc\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/sparc\/wp-json\/wp\/v2\/users\/64"}],"replies":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/sparc\/wp-json\/wp\/v2\/comments?post=19"}],"version-history":[{"count":0,"href":"https:\/\/scienceweb.clemson.edu\/sparc\/wp-json\/wp\/v2\/pages\/19\/revisions"}],"wp:attachment":[{"href":"https:\/\/scienceweb.clemson.edu\/sparc\/wp-json\/wp\/v2\/media?parent=19"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}