{"id":4554,"date":"2025-02-11T15:49:20","date_gmt":"2025-02-11T20:49:20","guid":{"rendered":"https:\/\/scienceweb.clemson.edu\/imms\/?p=4554"},"modified":"2025-02-11T15:49:20","modified_gmt":"2025-02-11T20:49:20","slug":"slim-based-high-resolution-ion-mobility-reveals-new-structural-insights-into-isomeric-vitamin-d-metabolites-and-their-isotopologues","status":"publish","type":"post","link":"https:\/\/scienceweb.clemson.edu\/imms\/slim-based-high-resolution-ion-mobility-reveals-new-structural-insights-into-isomeric-vitamin-d-metabolites-and-their-isotopologues\/","title":{"rendered":"SLIM-Based High-Resolution Ion Mobility Reveals New Structural Insights into Isomeric Vitamin D Metabolites and their Isotopologues"},"content":{"rendered":"<h1 class=\"article_header-title\"><span class=\"hlFld-Title\">SLIM-Based High-Resolution Ion Mobility Reveals New Structural Insights into Isomeric Vitamin D Metabolites and their Isotopologues<\/span><\/h1>\n<p><span class=\"hlFld-ContribAuthor\">Selena Kingsley<\/span><span class=\"comma-separator\">, Makenna Hoover, Terra Pettit-Bacovin, Anna Rose Sawyer,<\/span><span class=\"comma-separator\">\u00a0and <\/span><span class=\"hlFld-ContribAuthor\">Christopher D. Chouinard<\/span><strong>*<\/strong><\/p>\n<div class=\"article_header-cite-this\"><span class=\"cit-title\"><i>J. Amer. Soc. Mass Spectrom.<\/i><\/span>\u00a0<span class=\"cit-year-info\">2024<\/span><span class=\"cit-volume\">, 35<\/span><span class=\"cit-issue\">, 11, 2650-2658<\/span><\/div>\n<div class=\"article_header-epubdate\">\n<p><span class=\"pub-date epub-date\">Publication Date<\/span><span class=\"date-separator\">: May 6, 2024<\/span><\/p>\n<p><a href=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/jasms.4c00116\">https:\/\/pubs.acs.org\/doi\/full\/10.1021\/jasms.4c00116<\/a><\/div>\n<div><\/div>\n<div>Testing for vitamin D deficiency remains a high-volume clinical assay, much of which is done using mass spectrometry-based methods to alleviate challenges in selectivity associated with immunoassays. Ion mobility-mass spectrometry (IM-MS) has been proposed as a rapid alternative to traditional LC-MS\/MS methods, but understanding the structural ensemble that contributes to the ion mobility behavior of this molecular class is critical. Herein we demonstrate the first application of high-resolution Structures for Lossless Ion Manipulations (SLIM) IM separations of several groups of isomeric vitamin D metabolites. Despite previous IM studies of these molecules, the high resolving power of SLIM (<i>R<\/i><sub>p<\/sub>\u00a0\u223c 200) has revealed additional conformations for several of the compounds. The highly similar collision cross sections (CCS), some differing by as little as 0.7%, precluded adequate characterization with low-resolution IM techniques where, in some cases, wider than expected peak widths and\/or subtle shoulders may have hinted at their presence. Importantly, these newly resolved peaks often provided a unique mobility that could be used to separate isomers and provides potential for their use in quantification. Lastly, the contribution of isotopic labeling to arrival time distribution for commonly used\u00a0<sup>13<\/sup>C- and deuterium-labeled internal standards was explored. Minor shifts of \u223c0.2\u20130.3% were observed, and in some instances these shifts were specific to the conformer being measured (i.e., \u201cclosed\u201d vs \u201copen\u201d). Accounting for these shifts is important during raw data extraction to ensure reproducible peak area integration, which will be a critical consideration in future quantitative applications.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>SLIM-Based High-Resolution Ion Mobility Reveals New Structural Insights into Isomeric Vitamin D Metabolites and their Isotopologues Selena Kingsley, Makenna Hoover, Terra Pettit-Bacovin, Anna Rose Sawyer,\u00a0and Christopher D. Chouinard* J. Amer. Soc. Mass Spectrom.\u00a02024, 35, 11, 2650-2658 Publication Date: May 6, 2024 https:\/\/pubs.acs.org\/doi\/full\/10.1021\/jasms.4c00116 Testing for vitamin D deficiency remains a high-volume clinical assay, much of which&hellip;<\/p>\n","protected":false},"author":42,"featured_media":4555,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[98,115],"tags":[],"class_list":["post-4554","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-analytical-papers","category-news","category-98","category-115","description-off"],"_links":{"self":[{"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/posts\/4554","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/users\/42"}],"replies":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/comments?post=4554"}],"version-history":[{"count":0,"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/posts\/4554\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/media\/4555"}],"wp:attachment":[{"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/media?parent=4554"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/categories?post=4554"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/imms\/wp-json\/wp\/v2\/tags?post=4554"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}