{"id":4191,"date":"2024-07-18T14:32:03","date_gmt":"2024-07-18T18:32:03","guid":{"rendered":"https:\/\/scienceweb.clemson.edu\/cusoc\/?p=4191"},"modified":"2025-07-21T11:53:18","modified_gmt":"2025-07-21T15:53:18","slug":"newbiology-genetic-disease-investigator-a-case-study-for-sickle-cell","status":"publish","type":"post","link":"https:\/\/scienceweb.clemson.edu\/cusoc\/newbiology-genetic-disease-investigator-a-case-study-for-sickle-cell\/","title":{"rendered":"Biology: Genetic Disease Investigator \u2013 A Case Study for Sickle Cell"},"content":{"rendered":"

[vc_row][vc_column][vc_column_text css=””]**This class is limited to 32 students max.<\/p>\n

 <\/p>\n

In this lab, students will:<\/strong><\/p>\n

\u2022 Discuss DNA, genetic disease, homozygous and heterozygous alleles, Punnett squares, PCR, restriction enzymes, electrophoresis
\n\u2022 Set up a PCR reaction using forward and reverse primers and master mix
\n\u2022 Use a thermocycler to isolate and make copies of the gene of interest
\n\u2022 \u201cDigest\u201d PCR product using restriction enzymes
\n\u2022 Load \u201cdigested\u201d DNA on an electrophoresis gel
\n\u2022 Analyze gels for homozygous recessive, homozygous dominant, and heterozygous alleles.
\n\u2022 Operate micropipettes, vortex, and micro centrifuge<\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_btn title=”Make a Reservation HERE” style=”outline” shape=”square” color=”violet” align=”center” link=”url:https%3A%2F%2Fscienceweb.clemson.edu%2Fcusoc%2Fmake-a-reservation%2F|target:_blank”][\/vc_column][vc_column width=”1\/2″][vc_btn title=”Fees and Payment HERE” style=”outline” shape=”square” color=”violet” align=”center” link=”url:https%3A%2F%2Fscienceweb.clemson.edu%2Fcusoc%2Ffees-and-payment%2F|target:_blank”][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=””]Standards:<\/strong><\/p>\n

\u2022 B-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.
\n\u2022 B-LS3-2. Make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and\/or (3) mutations caused by environmental factors.
\n\u2022 B-LS3-3. Apply concepts of statistics and probability to explain the variation and distribution of expressed traits in a population<\/p>\n

Teaching words or concepts: adenine, allele, amino acid, base, cell, chromosome, cytosine, deoxyribose, DNA, dominant, double helix, enzyme, gene, gene mutation, , genetic code, genome, genotype, guanine, homozygous, hydrogen bond, mutation, nucleic acid, nucleotide, nucleus, offspring, parent, phenotype, phosphate, probability, Punnett square, RNA, recessive, replication, thymine, trait, transcription, translation[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Typical Schedule*:<\/strong><\/p>\n\n\n\n\n\n\n\n
9:00 \u2013 9:15<\/td>\nArrive\/walk to the lab<\/td>\n<\/tr>\n
9:30<\/td>\nStart lab (up to 64 students)<\/td>\n<\/tr>\n
11:30 \u2013 12:00<\/td>\nBreak for lunch (~1hr)<\/td>\n<\/tr>\n
12:30 \u2013 1:00<\/td>\nStart lab again<\/td>\n<\/tr>\n
1:30 \u2013 2:00<\/td>\nDepart<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

*Schedule can be adjusted to meet your school’s needs[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column][vc_media_grid grid_id=”vc_gid:1753113174210-ecc2d906-9ed3-9″ include=”3997,3998,4006″][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_column_text css=””]**This class is limited to 32 students max.   In this lab, students will: \u2022 Discuss DNA, genetic disease, homozygous and heterozygous alleles, Punnett squares, PCR, restriction enzymes, electrophoresis \u2022 Set up a PCR reaction using forward and reverse primers and master mix \u2022 Use a thermocycler to isolate and make copies of the…<\/p>\n","protected":false},"author":51,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[85,87],"tags":[],"class_list":["post-4191","post","type-post","status-publish","format-standard","hentry","category-for-school-groups","category-high-school","category-85","category-87","description-off"],"_links":{"self":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/posts\/4191","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/users\/51"}],"replies":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/comments?post=4191"}],"version-history":[{"count":0,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/posts\/4191\/revisions"}],"wp:attachment":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/media?parent=4191"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/categories?post=4191"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/tags?post=4191"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}