{"id":3720,"date":"2021-09-23T09:57:44","date_gmt":"2021-09-23T13:57:44","guid":{"rendered":"https:\/\/scienceweb.clemson.edu\/cusoc\/?p=3720"},"modified":"2025-07-21T11:54:45","modified_gmt":"2025-07-21T15:54:45","slug":"biology-mapping-plasmids-using-restriction-enzymes","status":"publish","type":"post","link":"https:\/\/scienceweb.clemson.edu\/cusoc\/biology-mapping-plasmids-using-restriction-enzymes\/","title":{"rendered":"Biology: Mapping Plasmids Using Restriction Enzymes"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text css=&#8221;&#8221;]**This class is limited to 32 students max.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>In this lab, students will:<\/strong><\/p>\n<ul>\n<li>Explore the structure and role of DNA<\/li>\n<li>Discover the origins and uses of restriction endonucleases (enzymes)<\/li>\n<li>Prepare restriction digests<\/li>\n<li>Analyze digested DNA fragments using gel electrophoresis<\/li>\n<li>Apply data to construct and verify plasmid maps<\/li>\n<\/ul>\n<p><!--more-->[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_btn title=&#8221;Make a Reservation HERE&#8221; style=&#8221;outline&#8221; shape=&#8221;square&#8221; color=&#8221;violet&#8221; align=&#8221;center&#8221; link=&#8221;url:https%3A%2F%2Fscienceweb.clemson.edu%2Fcusoc%2Fmake-a-reservation%2F|target:_blank&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_btn title=&#8221;Fees and Payment HERE&#8221; style=&#8221;outline&#8221; shape=&#8221;square&#8221; color=&#8221;violet&#8221; align=&#8221;center&#8221; link=&#8221;url:https%3A%2F%2Fscienceweb.clemson.edu%2Fcusoc%2Ffees-and-payment%2F|target:_blank&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<strong>AP Big Idea:<\/strong><\/p>\n<p>Big Idea 3:\u00a0 Information Storage and Transmission (IST)[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<strong>Typical Schedule*:<\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"312\">9:00 &#8211; 9:15<\/td>\n<td width=\"312\">Arrive\/walk to the lab<\/td>\n<\/tr>\n<tr>\n<td width=\"312\">9:30<\/td>\n<td width=\"312\">Start lab (up to 64 students)<\/td>\n<\/tr>\n<tr>\n<td width=\"312\">11:30 &#8211; 12:00<\/td>\n<td width=\"312\">Break for lunch (~1hr)<\/td>\n<\/tr>\n<tr>\n<td width=\"312\">12:30 &#8211; 1:00<\/td>\n<td width=\"312\">Start lab again<\/td>\n<\/tr>\n<tr>\n<td width=\"312\">1:30 &#8211; 2:00<\/td>\n<td width=\"312\">Depart<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>*Schedule can be adjusted to meet your school&#8217;s needs[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text css=&#8221;&#8221;]**This class is limited to 32 students max. &nbsp; In this lab, students will: Explore the structure and role of DNA Discover the origins and uses of restriction endonucleases (enzymes) Prepare restriction digests Analyze digested DNA fragments using gel electrophoresis Apply data to construct and verify plasmid maps<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[85,87],"tags":[],"class_list":["post-3720","post","type-post","status-publish","format-standard","hentry","category-for-school-groups","category-high-school","category-85","category-87","description-off"],"_links":{"self":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/posts\/3720","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/comments?post=3720"}],"version-history":[{"count":0,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/posts\/3720\/revisions"}],"wp:attachment":[{"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/media?parent=3720"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/categories?post=3720"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scienceweb.clemson.edu\/cusoc\/wp-json\/wp\/v2\/tags?post=3720"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}