In this lab, students will:

  • Collect quantitative data using a spectrophotometer.
  • Prepare dilutions using micropipettes.
  • Determine the wavelength of maximum absorbance for a specific dye.
  • Determine solution concentrations.
  • Analyze data by constructing graphs.
  • Apply data and graphs to find an unknown value.
  • Calculate the concentration of food dye in sports drink using Beer’s Law (AP, Honors).
  • Calculate percent error (AP, Honors).
  • Calculate the mass of dye in a sports drink (AP, Honors)


  • C-PS4-5. Communicate technical information about how some technological devices use the principles of the electromagnetic spectrum to cause matter to transmit and capture information and energy.

Typical Schedule*:

9:00 – 9:15 Arrive/walk to the lab
9:30 Start lab (up to 64 students)
11:30 – 12:00 Break for lunch (~1hr)
12:30 – 1:00 Start lab again
1:30 – 2:00 Depart

*Schedule can be adjusted to meet your school’s needs