In this lab, students will:

  • Transform bacteria using a plasmid containing a jellyfish gene.
  • Discover how bacteria transfer genetic material.
  • Predict results of transformation under specific conditions.
  • Discuss how and why genes are regulated.
  • Compare predictions to observations of non-transformed and transformed bacteria.
  • Learn about plasmids and how bacteria transfer genetic material.

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Standards:

  • B-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.
  • B-LS3-1. Ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions for characteristic traits passed from parents to offspring.
  • B-LS3-2. Make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors.
  • B-LS4-4. Construct an explanation based on evidence for how natural selection leads to the adaptation of populations.

 

AP Big Idea:

Big Idea 3:  Information Storage and Transmission (IST)

Typical Schedule*:

9:00 – 9:15 Arrive/walk to the lab
9:30 Start lab (up to 64 students**)
10:45 – 11:15 End lab

*Schedule can be adjusted to meet your school’s needs
**If you have more than 64 students, see large group schedule

Typical Large Group Schedule*:

9:00 – 9:15 Arrive/walk to the lab
9:30 – 11:00 Lab for A group** (up to 64 students)
11:00 – 12:00 Switch groups
12:00 – 1:30 Lab for B group*** (up to 64 students)
1:30 Depart

*Schedule can be adjusted to meet your school’s needs
**B group participates in another campus activity; school chaperones arrange
***A group participates in another campus activity; school chaperones arrange