In this lab, students will:

• Discuss mixtures vs solutions, chemical vs physical properties, separation of mixtures, types of chromatography, gel electrophoresis
• Extract dyes from various candies
• Pour and load a practice gel
• Load extracted dyes onto an electrophoresis gel
• Analyze gels to:
Classify dyes as pure solutions or mixtures
Rank the dyes in order of increasing molecular size
• Separate the dyes in Kool-Aid by using column chromatography:
Load the Kool-Aid on the column
Load solvents of increasing polarity on the column to separate dyes
Classify the dyes according to increasing polarity
• Utilize micropipettes, gel electrophoresis equipment, chromatography columns

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Standard:

  • C-PS1-3.  Plan and conduct an investigation to gather evidence to compare the structure of substances at a bulk scale to infer the strength of various forces between particles.               PS1.A: Structure and Properties of Matter The structure and interactions of matter

                    at the broader level are determined by various forces within and between atoms

    C-PS2-6. Communicate scientific and technical information about why the molecular structure determines the functioning of designed materials.

     

    Teaching words or concepts:  chemical property, mass, mixture, polar, nonpolar, polarity, intermolecular attraction, physical property, soluble, solution, solvent.

Typical Schedule*:

9:00 – 9:15 Arrive/walk to the lab
9:30 Start lab (up to 64 students)
11:30 – 12:00 Break for lunch (~1hr)
12:30 – 1:00 Start lab again
1:30 – 2:00 Depart

*Schedule can be adjusted to meet your school’s needs